goat anti cd31  (R&D Systems)

Journal: eLife

Article Title: Interleukin-33 regulates the endoplasmic reticulum stress of human myometrium via an influx of calcium during initiation of labor

doi: 10.7554/eLife.75072

Figure Lengend Snippet: Compared with the lipopolysaccharide (LPS) group, the expression of IL-8 and IL-6 was increased in the LPS + IL-33 siRNA group and decreased in the LPS + BAPTA AM group ( n = 5). The data are presented as mean percentages of control ± standard deviation (SD). Statistical analyses were compared using a one-way analysis of variance and Bonferroni multiple comparisons test. *p＜0.05 when compared to controls.

Article Snippet: Primary antibody incubation was performed overnight at 4°C with goat monoclonal anti-IL-33 antibody (1:200, MAB36253, R&D) diluted in 0.2% BSA in PBS.

Techniques: Expressing, Standard Deviation

Journal: eLife

Article Title: Interleukin-33 regulates the endoplasmic reticulum stress of human myometrium via an influx of calcium during initiation of labor

doi: 10.7554/eLife.75072

Figure Lengend Snippet: Diagrammatic model for the role of interleukin-33 (IL-33) in the myometrium where it participates in maintaining a uterine quiescent state at the tissue-to-cellular level during late pregnancy.

Article Snippet: Primary antibody incubation was performed overnight at 4°C with goat monoclonal anti-IL-33 antibody (1:200, MAB36253, R&D) diluted in 0.2% BSA in PBS.

Techniques:

Journal: eLife

Article Title: Interleukin-33 regulates the endoplasmic reticulum stress of human myometrium via an influx of calcium during initiation of labor

doi: 10.7554/eLife.75072

Figure Lengend Snippet: Compared with the lipopolysaccharide (LPS) group, the expression of IL-8 and IL-6 was increased in the LPS + IL-33 siRNA group and decreased in the LPS + BAPTA AM group ( n = 5). The data are presented as mean percentages of control ± standard deviation (SD). Statistical analyses were compared using a one-way analysis of variance and Bonferroni multiple comparisons test. *p＜0.05 when compared to controls.

Article Snippet: Then samples were incubated overnight at 4℃ with goat anti-human IL-33 monoclonal antibody (MAB36253, R&D) at a concentration of 0.1 µg/ml and then one to three drops of biotinylated secondary antibodies for 2 hr at room temperature.

Techniques: Expressing, Standard Deviation

Journal: eLife

Article Title: Interleukin-33 regulates the endoplasmic reticulum stress of human myometrium via an influx of calcium during initiation of labor

doi: 10.7554/eLife.75072

Figure Lengend Snippet: Diagrammatic model for the role of interleukin-33 (IL-33) in the myometrium where it participates in maintaining a uterine quiescent state at the tissue-to-cellular level during late pregnancy.

Article Snippet: Then samples were incubated overnight at 4℃ with goat anti-human IL-33 monoclonal antibody (MAB36253, R&D) at a concentration of 0.1 µg/ml and then one to three drops of biotinylated secondary antibodies for 2 hr at room temperature.

Techniques: